Course Description
Fundamental molecular processes:
A) Atoms and molecules, intermolecular and intramolecular interactions, covalent bonds, ionic bond, hydrogen bond. Weak molecular interactions and relevance in molecular biology.
B) Structure of nucleic acids and nucleotides: Structural organization of DNA(Watson-Crick model) Characteristic features of A,B, C and Z DNA. Structural organization of tRNA and micro RNA stability of proteins and nucleic acids.
C) Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons).
C) DNA replication, repair and recombination (Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, extra chromosomal replicons, DNA damage and repair mechanisms, homologous and site-specific recombination).
D) RNA synthesis and processing (transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases, capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, structure and function of different types of RNA, RNA transport).
E) Protein synthesis and processing (Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, Post- translational modification of proteins).
F) Control of gene expression at transcription and translation level (regulating the expression of phages, viruses, prokaryotic and eukaryotic genes, role of chromatin in gene expression and gene silencing).
Methods In Molecular Biology and Genomics:
- Isolation and purification of RNA , DNA (genomic and plasmid) and proteins, different separation methods.
- Analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis, Isoelectric focusing gels.
- Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems.
- Expression of recombinant proteins using bacterial, animal and plant vectors.
- Isolation of specific nucleic acid sequences, Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors.
- In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms.
- DNA sequencing methods, strategies for genome sequencing. Methods for analysis of gene expression at RNA and protein level, large scale expression, such as micro array based techniques RFLP, RAPD and AFLP techniques
Who This Course is for
Our molecular biology course can prepare students for a wide range of competitive exams and PG exams in Biological sciences. The course offers you detailed classes on all aspects of molecular biology, providing you in depth knowledge of all basic molecular process.
